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For inducible expression regarding GSK3�� in MII tissue, siRNA-insensitive GSK3�� alternatives had been subcloned in the doxycycline-inducible retroviral appearance vector (pSTC-Puro), along with retroviral debris were used to be able to transduce MII-rtTA cellular material. cDNAs for man ��-catenin, wild-type and phospho mutant (S33/37/41A, akin to your GSK3 internet sites along with the CK1 prim

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