s. PBMC have been isolated by Ficoll-Hypaque density gradient centrifugation. Cells had been suspended in complete RPMI-8 supplemented with 8% heat-inactivated fetal calf serum ). Within the experiment treating cells with adenosine, serum-free medium X-VIVOTM 20 was utilised. To purify human naive and memory CD4 T cells from PBMC, fresh PBMC had been stained with antibodies certain for cell surfac


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