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Al lung tissues and five of seven NSCLC tissues. As a result, asAl lung tissues and 5 of 7 NSCLC tissues. As a result, as with the Ex4a(+)WT1 isoform in regular cells, in three of 7 paired samples, typical lung tissues expressed the Ex4a(+)WT1 isoform at amounts corresponding to individuals in lung cancer tissues. Apparently, during the remaining four paired samples, standard lung tissues ex
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Chemical reagentsDoxorubicin (Dox, Sigma Chemical Co., Steinheim, Germany) and Etoposide (WAKO, Osaka, Japan) have been accustomed to induce apoptosis. Cells ended up developed to eighty confluence, handled with Dox in the indicated concentrations for twelve h, and afterwards harvested. Puromycin (Invitrogen, Carlsbad, CA) was utilized to block nonsense mediated mRNA decay (NMD) pathway [50]. Cel
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Chemical reagentsDoxorubicin (Dox, Sigma Chemical Co., Steinheim, Germany) and Etoposide (WAKO, Osaka, Japan) have been accustomed to induce apoptosis. Cells ended up developed to eighty confluence, handled with Dox in the indicated concentrations for twelve h, and afterwards harvested. Puromycin (Invitrogen, Carlsbad, CA) was utilized to block nonsense mediated mRNA decay (NMD) pathway [50]. Cel
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